Sentence examples for are at a confluence from inspiring English sources

But we are at a confluence now.

Media was changed every 2 days and cells were passaged at a confluence of 80-90% performing trypsinization (Invitrogen).

Cells were split at a confluence of 80%.

For intracellular Ca2+ measurement, cells were plated at a confluence of 80 90% in clear-bottom poly- d-lysine coated 96-well plates (Corning, NY, USA) for 24 h prior to experiments.

HEK-293 cells were plated at a confluence of 50 60% in 12-well plates (2.4-cm diameter per/well) and transfected with wild-type or various mutant forms of full-length FLAG-tagged human KCCs.

To generate HeLa TAPBPR KO cell lines, cells were seeded at a confluence of 80% in 6 well format and transfected with TAPBPR-CRISPR plasmids in the absence of serum using Lipofectamine2000 (Invitrogen, Thermo Fisher Scientific).

For the microarray, the cells were seeded at a confluence of 60%% in 6-cm plates, starved for 2 days in 5 % CDFBS-containing medium, followed by 1 h or 6 h compound stimulation (10 nM) in serum-free medium.

As shown before for a number of cell types (reviewed in [ 17]), high cell density caused an increase in Stat3-ptyr705 levels in all lines (e.g. Figure 5A, lanes 1-4 and 5-8), therefore Stat3-ptyr705 levels were assessed at a confluence of 50% for this experiment (see Methods).

H1299 cells were transfected at a confluence of 90% in 6 wells plates, with 2.5 μg of the firefly luciferase reporter plasmid (p21 waf-1-luc or Hdm2 in pGL3 vector; Promega), 0.5 μg of a plasmid expressing either human p53 (pLPcontainingaining the CMV promoter), axolotl p53 (FG12/CMV-Ap53 FG12/CMV-Ap53as cortrol.

It's at the confluence of the Lochsa and the Selway.

At the time of transfection cells were at 60% confluence.