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Disease severity was globally evaluated by the Shwachman-Kulczycki score, in which four separate aspects are assessed: activity, physical examination, nutrition and chest roentgenogram.
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An important challenge for the National Children's Study is assessing activity patterns among mothers, infants, and young children.
Locomotor activity was assessed using activity cages, where the mouse can move inside a cylindrical corridor.
The effect of GSSG on enzyme activity was assessed by activity measurement following incubation of de-glutathionylated enzyme with increasing concentration of GSSG.
Open field activity was assessed using activity monitor software (Med Associates Inc., Georgia, Vermont) as previously described (21).
Sleep was assessed using activity monitors and self-reported sleep diaries.
Activity was assessed by UCLA activity score and a specifically designed activity score.
ROCK2 activity was assessed by ROCK Activity kit (Cell Biolabs, San Diego, CA, USA).
Physical activity was assessed with an activity frequency questionnaire based on the Arizona Activity Frequency Questionnaire [ 23].
Physical activity will be assessed with the Activity Questionnaire for Adolescents & Adults (AQUA) [ 31].
Physical activity level is assessed with an activity monitor (GT3X + Tri-Axis Actigraphy Monitor, ActiGraph®).
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