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Samples are assayed using highly sensitive enzyme immunoassays.
Type II loci are assayed using a single probe, with Meth and Unmeth signals derived from the green and red channels respectively.
All genetic markers that are assayed using fragment size genotyping are subject to erroneous inferences if the sizes do not reflect similarity of the underlying nucleotide sequence.
Gene Ontology terms will be provided whenever functional significance of the nodes or edges are assayed using inhibitors, mutants, knockouts or silencing studies.
(E ) Buffer control or affinity-purified DKC1 RNPs from salt-eluted Poros-HQ fractions over a threefold concentration range are assayed using in vitro transcription.
Aggrecan degradation products are assayed using antibodies 846, 3B3, and 7D4 that detect chondroitin sulfate neoepitopes, 5D4 that detects keratan sulfate epitopes, and the VIDIPEN and NITEGE antibodies that recognize aggrecanase and MMP cleavage sites, respectively, within the interglobular G1 domain of aggrecan [ 33].
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Enzyme activity was assayed using 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate 2,2′-azino-bis 3-ethylthiazoline-6-sulfonate
The samples were assayed using the plaque forming technique.
Ninety-six hours post transfection, cell viability was assayed using CellTiter‐Glo (Promega) quantifying cellular ATP content.
The expression of specific mRNAs was assayed using fluorescence-based real-time quantitative PCR (qPCR).
The activities were assayed using malachite green, which allows photometric determination of the Pi concentrations.
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