Two neurophysiological parameters are ascertained using an EEG amplifier (19 electrodes, 10 20 system): a) Quantitative EEG (QEEG) is recorded during 6 minutes resting condition (3 minutes eyes closed and 3 minutes eyes open) and during the first, last and follow-up training session, with the same equipment used for feedback but at 19 electrodes.
The urinary and plasma biomarker performance was ascertained using a receiver operating characteristic (ROC) curve analysis.
As explained in the section describing "Motion classification", the SVM classification performance depends on selection of kernel parameter (upgamma) and penalty parameter C. The optimal combination of (upgamma) and C was ascertained using a grid search within the training data.
Using a case control design, MVC deaths were ascertained using a military mortality registry, and an integrated health system database provided information on health system eligibility, pharmacy transactions, and medical encounters.
The sequence and minor groove specificity of the achiral compounds was ascertained using a Taq DNA polymerase stop assay and a thermal induced DNA cleavage experiment using either a fragment of pBR322 or pUC18 plasmid DNA.
In this study, all types of medical care costs (not only those related to headache) were derived from the claims records of a large health plan, whereas diagnostic status (migraine or not) and comorbid and demographic status was ascertained using a telephone interview among members of the health plan [35].
β2AR expression was ascertained using a highly quantitative 125I-cyanopindolol (125I-CYP) radioligand binding assay.
Proper placement of the epidural catheter was ascertained using a 3-mL test dose of lidocaine 1% to 2% with epinephrine 1∶200000.
Equal parts cell suspension and 0.4% trypan blue (Sigma-Aldrich, St . Louis MO, USA) were mixed immediately before cell viability was ascertained using a Bright Line hemocytometer (Reichert, Buffalo, NY, USA) and Nikon TMS phase-contrast microscope (Nikon, Tokyo, Japan).
Disease history was ascertained using a questionnaire.
