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We then designed a novel cell-free cytoskeleton binding assay in which a set of GST LSP1 fusion proteins are allowed to bind to the cytoskeleton in NP-40 soluble lysates of BW5147 cells and are recovered in the low-speed detergent insoluble pellet.
In magnetophoretic separation, biochemically functionalized magnetic beads are allowed to bind selectively to target analytes, which are then separated from the background stream using a suitably imposed magnetic field.
Note that the players are allowed to bind an agreement only on the space of feasible outcomes.
Only when all the riboswitches' aptamers are on, ribosomes are allowed to bind the mRNA and to start translation.
The effect of disruption of the binding site can be measured by determining protein function after mAbs are allowed to bind their epitopes.
In this system, compounds introduced by a carrier solution are allowed to bind to the ERα LBD in a first reaction coil, and the E2-F tracer is introduced after this coil.
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The ligand binding assay was a variant of the ELISA in which dilutions of ligand (rhFGF-2) were allowed to bind to a ligand binding protein such as rhPln.D1 that had been coated onto the wells in PBS-N3.
32P-labeled (1 pmol, 20,000 cpms) IDX-rasISS1 RNA was allowed to bind to 640 ng recombinant hnRNP H in binding buffer (1 mM ATP, 1.7 mM MgCl2 and 0.1 µM heparin in 1×Roeder D buffer).
After addition of a binding buffer and the magnetic particles (Siemens Healthcare Diagnostics, Cologne, Germany) nucleic acids were allowed to bind to the particles for 15 min at room temperature.
Antibody was allowed to bind for 20 minutes at 4 °C.
The supernatant was allowed to bind to the column overnight at 4 °C.
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