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Usually, human blastocyst is mimicked by spheroids of placental cells that are allowed to attach to monolayer cultures of endometrial cells or 3D matrices.
It is demonstrated above, that round cells (both mitotic and trypsinized) that are allowed to attach and spread to the substratum exhibit membrane blebs and active signal transduction proteins.
This implicates that the formation of membrane blebs is related to attaching and spreading of cells, rather than to a specific cell cycle phase, and as such this phenomenon may be expected to occur in trypsinized cells that are allowed to attach and spread regardless the cell cycle phase.
Some email providers limit the size and type of file you are allowed to attach..EXE and executable files are quite often rejected by common email providers.
Similar(56)
The floating EBs were allowed to attach on tissue culture dishes and fixed at different days post-attachment.
Englishness must never be allowed to attach itself to ethnicity.
When a group takes money from the government, the government is allowed to attach strings.
It's their inquiry into working practices at Sports Direct and witnesses can't be allowed to attach conditions to their appearance.
Cells were allowed to attach overnight and then were treated with different concentrations of compounds.
Cells were allowed to attach and grow to ~80 90% confluency (density) for cytotoxicity testing.
Cells were allowed to attach the surface for 24 h prior to treatment.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com