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For three samples collected at 0 h, 2 h and 24 h, 95.3%, 95.3% and 95.5% of the reads can be aligned back to the 74,797 contigs, and 61.6%, 61.7% and 63.6% were aligned concordantly exactly 1 time, respectively.
"The stars are aligned".
The planets are aligned.
"All the stars are aligned".
Our interests are aligned!
The military are aligned with the gentry.
Your interests are aligned.
Yet powerful forces are aligning against cash.
Here a read pair is said to be concordantly mapped to the reference genome when the end with a lower mapping coordinate is aligned to the forward strand, the end with the higher mapping coordinate is aligned to the reverse strand (i.e., FR read pairs, where F and R refer to forward and reverse strands, respectively) and the distance between the mapped ends is within an expected range.
Doors should be aligned.
"Finally everything is aligned".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com