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Expanded view (middle) of the linker domains that are aligned based on the stretch (gray box) for which the sequence alignment is shown on the right.
Here, we introduce and evaluate fragment oriented molecular shapes (FOMS), where shapes are aligned based on molecular fragments.
These protein-coding sequences are aligned based on translated protein sequences using Clustal W program implemented in BioEdit [ 23, 24].
In particular, we find protein pairs with low sequence similarity for which the interaction similarity strengthens the statistical inference of homology, as well as protein pairs without sequence similarity, which are aligned based on their interactions alone.
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The codeml program of the PAML software package was used to detect positive selection in the CYP71C subfamily [ 39]. cDNA sequences were aligned based on protein alignment by using the RevTrans 1.4 server [ 40].
Nucleotide sequences of protein-coding genes were aligned based upon amino-acid alignments using CodonAlign [75].
The DNA sequences were aligned based on the protein alignment.
Nucleotide sequences were aligned based on amino acid alignments using MUSCLE 3.6 [ 88].
The nucleotide sequences were aligned based on amino acid alignment using PRANK by applying the TranslatorX perl script [ 43].
The protein-coding DNA sequences were aligned based on their protein alignments at the web server http://www.bork.embl.de/pal2nal/[ 27].
Protein-coding DNA sequences (CDS) were aligned based on the protein alignments in the DAMBE with the default parameters, then converted the CDS alignments into PAML format for further analyses [ 67, 68].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com