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Keep at least 1 m away from the vibrator, and wait until the seeds are aligned automatically (about 40 s).
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Sequences were aligned automatically using ClustallW [ 30], and the alignment was then refined by eye using MEGA 4 [ 31].
SPECT and CT images were aligned automatically by dedicated software in the SPECT/CT scanner.
Predicted amino acid sequences were aligned automatically using ClustalW [ 32] with manual correction in Seaview [ 33].
Accession numbers are listed by species in Additional file 1. Allele sequences were aligned automatically using MAFFT v6 [ 67, 68], and corrected by eye in MacCladev4.08 [ 69].
Sequences were aligned automatically using MUSCLE [ 21] implemented with default settings in SeaView v4.2.12 [ 22], and adjusted manually in BioEdit v7.1.11 [ 23].
The sequences were aligned automatically by using Clustal X software (http://www-igbmc.u-strasbg.fr/BioInfo/ClustalX) and then manually with MacCLADE version 4.05 (http://macclade.org/macclade.html) (10 ).
The results of the phantom experiment showed that by using the calibrated system to track surface features in 3D, the multispectral image stack could be aligned automatically despite significant movement of the subject during the acquisition process and varying visibility of salient features in the multispectral channel.
For each database search, sequences that score more than the family-specific threshold are aligned to the HMM profile automatically to make a full alignment.
The images that are used for generating 3D point clouds are automatically aligned with BIM (see Fig. 1) after their 3D point clouds are aligned with BIM either manually or automatically.
"The stars are aligned".
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CEO of Professional Science Editing for Scientists @ prosciediting.com