This suggests that the sequences in the selectable marker had been affecting the expression of the EGFP.
Since the number of genes tested was not exhaustive, Bcor may be affecting the expression of other genes important for endoderm development that are yet unidentified.
Given that Cyclin B1 and Securin protein levels were down-regulated, we next investigated if inhibition of transcription was affecting the expression of cyclin B1 and Pttg (the gene that codes for Securin) genes.
As shown in Figure 4B, treatment of myotubes with TWEAK for 18h did not affect the transcript levels of Dicer, Dorsha, or Exportin-5 indicating that TWEAK may be affecting the expression of miRs but not their processing from pre-miRs to mature miRs.
The relatively short intergenic region (796 bp) and the close proximity of the proximal breakpoint to the initiation codon of Dlh (118 bp) suggest that the inversion might be affecting the expression of Dlh and/or Mdp, a question that deserves further work.
We next investigated whether the upregulated expression of TFs was affecting the expression of their target genes.
We also cannot discard the possibility that DNA methylation is affecting the expression of a co-regulator of CTCF, such as the poly-ADP-ribosyl polymerase 1, among others [ 32].
It is worth to mention, however, that the hormones may be affecting the expression of other clock genes, such as Per2 or Bmal1, or even exerting a posttranslational effect.
Similarly large decreases in transcript abundance were also observed for β-gal and scFv13-R4 (Fig. 2A), suggesting that the same mechanism was affecting the expression of these proteins as well.
From these data it is clear that the spf deletion is affecting the expression of a relatively large number of genes (e.g., 385 genes are ≥ 2 fold differentially expressed after addition of glucose) that belong to a wide range of categories, such as "foreign DNA", "carbohydrate metabolism", "cell envelope", "transport proteins", "motility", "iron homeostasis", and "quorum sensing".
Due to the direct association found between P-cadherin and HIF-1α expression in invasive breast carcinomas, as well as to the described association between both markers and the maintenance of stem-like properties, we decided to evaluate if HIF-1α stabilization could be affecting the expression of P-cadherin in breast cancer cells.
