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Chemicals are added to convert ammonium ions into ammonia gas.
Nitrite and N-naphthylethylene diamine are added to convert RFAP to its pink azo-dye derivative, which absorbs strongly at 562 nm.
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Next superoxide dismutase was added to convert any superoxide to H2O2.
After further incubation at 37 °C for 10 min, 0.8 ml 2.5 M NaOH was added to convert prephenate formed in the enzymatic reaction to phenyl pyruvate.
Another convenience function was added to convert a Python list to a C array of doubles, as this type of input is required for a small number of OpenBabel functions.
Nigericin (80 µn/ml) was added to convert the pH component of Δp into mV units.
Superoxide dismutase (SOD) (90 U/ml) was added to convert the produced superoxide to H2O2.
Then, thio-NAD+, the oxidized form of β-thionlactinamide adenine dinucleotide, was added to convert thio-NAD+ to thio-NADH.
After depletion of ATP, 50 μl of Kinase Detection Reagent was added to convert ADP to ATP and introduce luciferase and luciferin to detect ATP.
For the analysis of δCDIC-PPP, a 2 ml helium (He) headspace was created, and H3PO4 was added to convert all DIC species to CO2.
Reactions were prepared as described for the NADH consumption assay except that excess C23O was added to convert catechol to 2-hydroxymuconic semialdehyde (ε410 = 12 620 M–12cm–12.
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