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The active sites of CifPl and CifBp are shown to contain reactive cysteines, which are accessible to modification by a thiol-reactive compound.
The ParM construct contains two exposed cysteine residues (Cys and Cys) that are accessible to modification with thiol-reactive iodoacetamide probes.
The ParM construct described here (His6/K33A/D63C/T174A/T175N/D224C/C287A) contains two exposed cysteine residues (Cys and Cys) that, according to the known crystal structures, are accessible to modification; indeed, they were added for that purpose.
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(4) hPSCs are accessible to genetic modification, which are also present in the respective hPSC-derived cardiomyocytes.
Histone complexes possess flexible N terminal tails that are accessible to posttranslational modifications and can strongly impact the functional capacities of nucleosomes [41].
Moreover, the N-termini of histone proteins contain multiple lysine residues and are accessible to covalent modifications such as acetylation, methylation, sumoylation, biotinylation, phosphorylation, glycosylation, and ADP-ribosylation, thus allowing regulation of gene transcription (Fig. 1) [ 4, 5, 33– 33].
Also, since these resources are accessible to a few people, it will be very easy to discover and detect any modification and the person that is responsible for the modification.
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