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Stirred vessel bioreactors were chosen since they are (a) suitable for large scale culture (Nienow, 2006); (b) used routinely in industry; and (c) have been used in recent studies with mESCs (Abranches et al., 2007; Fernandes et al., 2007; Fok and Zandstra, 2005) and hESCs (Fernandes et al., 2009; Oh et al., 2009).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com