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A specimen of the deep-sea fish Ebinania brephocephala collected by trawl net was kept alive under pressure in the pressure-stat aquarium system for 64 days.
A novel pressure-stat aquarium system has been developed for the study of living deep-sea multicellular organisms under high hydrostatic pressure.
The aquarium system was controlled at 26°C with a photoperiod of 14 h/10 h light/dark cycle.
Fish were kept in a common re-circulating aquarium system with controlled temperature and salinity of 20°C, 15 ppt salinity for one year before experiments.
Water conditioning and environmental quality was maintained according to aquarium system use and care manual and The Zebrafish Book (Westerfield 2000).
Medaka (Oryzias lapties), HdrR strain, were bred and kept in a freshwater (FW) recirculating aquarium system in the Atmosphere and Ocean Research Institute, The University of Tokyo.
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Unlike typical aquarium systems, it uses no chemical filters.
Writer spoke with Edmund J. Mowka, Jr. who is with the research-and-development section of Aquarium Systems, of Mentor, Ohio.
Those and other species are also economically important as aquarium plants, where their fast growth and high oxygen production can contribute to the health of aquarium systems.
The system was continuously aerated by a low flux pump normally used in aquarium systems.
In the lab, nematodes were removed from the sediment by decantation, and the nematodes were rinsed off a 38 µm sieve with artificial sea water (Instant Ocean® salt, Aquarium Systems, France).
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