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We analyze the emission current as a function of s from near close packed (s ≥ 0.25 h) to s = 10 h (approximately isolated tubes).
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Recovery of colonies was efficient: approximately 1 colony could be isolated from approximately 13 cells deposited (clonal efficiency of 7.7%).
In particular, ST1 corresponds to strains isolated approximately 60 years apart (SH-PERM and UR.BQ.MNHP 295).
Of the blastomeres isolated, approximately 21% (8 out of 38) divided once, but no blastocele could be detected in arrested parthenotes.
In the case of MATE, approximately 80% was isolated.
In initial experiments we isolated approximately 25 ng of total RNA from each sample.
Approximately 90% pure isolated cardiomyocytes were confirmed by staining with sarcomeric α-actinin antibody (Abcam, Cambridge, MA, USA).
Approximately 2,670 SSRs isolated from our genomic libraries were subjected to polymorphism detection, including 518 SSRs that had been mapped [ 7].
Phylogenetic analysis of the strains shows a close relationship to Swedish and Russian SINV strains, isolated approximately 20 years ago from mosquitoes.
Briefly, the pooled Tγ−X product (approximately 20 pmol) isolated by reversed-phase HPLC (see above) was adsorbed to the wells by incubating at 25 °C for 1.5 h.
This study isolated approximately 180,000 ESTs, and of these, 1,900 (1.0%) mapped to the human genome in regions not previously annotated in the Ensembl database.
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