The concept proposed should have applicability to other two-phase alloys containing approximately equal volume fractions of each phase.
A duplex stainless steel with approximately equal volume fractions of ferrite and austenite was processed by high-pressure torsion.
Approximately an equal volume of acid-washed glass beads was added for efficient cell disruption.
Briefly, serial twofold dilutions of inactivated sera (starting at 1 10) were mixed with an equal volume of approximately 30 PFU DENV1-4 and ImDENV2 in DMEM containing 2 % FBS for 2 h at 37 °C.
A ductile, silvery white metal usually with dull lustre owing to a surface film of aluminum oxide, aluminum is light, weighing approximately one-third as much as an equal volume of copper or steel.
Subsequently, the blood was centrifuged at 16,000×g at 4 °C for 2 min, and the plasma (approximately 100 μl) was vortexed with an equal volume of acetonitrile.
An equal volume of virus, adjusted to approximately 8HAunits/50 microliter was added to each well.
The antigen and antibody were allowed to incubate for approximately 15 minutes prior to the addition of an equal volume of 0.5% guinea pig red blood cells.
Furthermore, so long as water can move sufficiently rapidly between plasma and ISF that the osmolalities of these fluids are approximately equal, the volume of ISF in the brain is determined by the solute content of the ISF and the osmolality of the plasma.
One hundred microliters of freshly prepared HRP substrate was added to each well, and the plates were incubated at room temperature for approximately 5 min. The reaction was stopped with an equal volume of 1 N sulfuric acid.
