All transferred plasmids were stably propagated in the recipient strain without selective pressure for approximately 66 generations.
have been approximately 80 generations of members since the "Golden Days" six and 1/4 inch achromat of 80 inch focal length (approximately f/15).
From the emergence of the human genus, Homo, about 2.4 million years ago, our ancestors survived as hunter-gatherers for approximately 84,000 generations.
Finally, after approximately 300 generations, single colonies were obtained, and the best isolated strain showed a lag phase of 17 h instead of 90 h for parental strain in media supplemented with 17 mM furfural.
The average lifetime of a RAS member is about two years, so there have been approximately 80 generations of members since the "Golden Days" of Sagan & Co. in the early 1950's". Because of this, nearly all information retained about the RAS is in the form of logbooks.
More moderate fitness costs (≤5% per insert) can result in transgene frequencies approaching fixation in approximately 20 generations (Figure 4).
After approximately 50 generations, the culture was diluted and spread on YES medium added with or without glucosamine, respectively.
Stationarity was apparently reached by approximately 106 generations, and the last 3×106 generations from each independent run were (conservatively) used to assemble the phylogeny posterior distribution.
This process was repeated at five successive growth intervals approximately 25 generations apart using single colonies that arose on the YPD viability plates.
During growth in the absence of selective pressure (sakacin P), the sakacin P resistant phenotype in L502-1 storeverto revert after approximately 20 generations.
