Exact(4)
The main evidence: Mr. Ebershoff has fractured his narrative into texts, memoirs, depositions, letters, newspaper articles, an ersatz Wikipedia entry and even a supposed approximate transcript of conversation between wives of Brigham Young, the 19th-century Mormon patriarch.
The average CT was converted to an approximate transcript number by the equation n = 2(40−Ct).
This method allows the determination of approximate transcript sizes.
Thus for most genes, transcript levels in whole filaments closely approximate transcript levels in vegetative cells.
Similar(56)
An identical approach was also taken to approximate the transcript coverage with the difference that the number of all identified AGIs was not recorded but the number of AGIs that was identified at least by 5, 10 and 100 reads.
As RNAseq mapping provides only approximate definition of transcript ends, we searched for a promoter motif within an area +/− 250 bp from the point at which 5' short-read coverage for exon 1 ceased.
Furthermore, the demonstration that whole transcriptome sequencing can be used to identify candidate splicing events and approximate primary microRNA transcript coordinates represents a significant step towards the large-scale elucidation of mechanisms regulating the expression of microRNAs at the transcriptional and post-transcriptional levels.
To empirically validate the normalization protocol that better approximates changes in transcript levels, we used quantitative RT-PCR as an independent method to measure transcript level changes of more than 30 different mRNAs (see below).
Similarity searches were carried out using the hybrid assembly resulting from the TGICL pipeline (OakContigV1) that provides an approximate estimate of unique transcripts, because it discriminates alternative spliced transcripts.
We consider RNA-seq assays independently, computing an approximate posterior for the transcript proportions θ in each assay.
Hence, most of them represent a fragment of a full transcript (the approximate mean length of a V2R coding sequence in vertebrates is 2,100 bp).
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