Thus, read number can reliably be assumed to approximate relative template abundance.
PCR reactions were carried out in 25 μl reaction mixtures containing 18.8 μl of sterile distilled H2O, 2.5 μl of 10× LA PCR buffer II (Mg2+ plus, Takara), 0.5 μl of dNTP (10 mM each), 1 μl of each primer (5 μM), 0.2 μl of LA Taq polymerase (5 unit/μl, Takara), and 1 μl of DNA template (approximate 30 ng).
To circumvent this constraint, our technique starts with a training stage, where several high-SNR EPSCs are identified by a human operator and fitted by polynomial models to build an array (bank) of approximate matched filters (templates).
Six samples with differing InGaN active regions were investigated, separated into two sets: three samples contained QDs formed by "droplet epitaxy" grown on pseudosubstrates of varying dislocation density; and three QW samples with differing growth conditions were grown on low-dislocation templates (approximate density (3.0 ± 0.3) × 10 cm–2).
To approximate interface curvature, a template method is employed which incorporates crystalline anisotropy.
After that, the model with optimum shape and period parameters is taken as a template to approximate the impulses in faulty bearing signal.
Each participant's VFM map was then nonlinearly aligned to a common, study-specific template in approximate MNI space using the Advanced Normalization Tools software package [Avants et al., 2008] and smoothed with a 3 mm Gaussian kernel.
This template shows approximate characters for them in the following way: {{keypress|Win}} → ⊞ Win.
Anatomical regions are defined in MNI coordinates (following normalisation of each participant's data to the ICBM-152 template), which closely approximates to the space described by Talairach and Tournoux [32].
Functional data were then transformed into a standard anatomical space (3-mm isotropic voxels) based on the ICBM 152 brain template (Montreal Neurological Institute) which approximates Talairach and Tournoux's (Talairach and Tournoux 1988) atlas space.
