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Each probe was re-annotated to the latest approved symbol if an unambiguous mapping (i.e. single symbol mapping to approved symbol) could be determined, else the original gene name was maintained.
All gene symbols were then checked with HGNChelper package [ 40] and updated to the latest approved symbol if necessary.
We matched the tagged gene names against the approved symbol, approved name, previous symbols, previous names, aliases and name aliases fields of the database.
Constructs harbouring human PIP4K2 (HGNC approved symbol, previously PIP5K2) genes [ 22] were used to generate PCR fragments that were subcloned into the pGEX6P vector (GE Healthcare).
hESCs (human ESCs) are similar to mESCs in that S-phase is highly populated (~50% of cells) and cyclin E (HUGO approved symbol CCNE) expression does not display periodicity, but is constitutive [ 8].
Geminin (HUGO approved symbol GMNN) is down-regulated in trophoblast giant cells and the ablation of geminin is sufficient to cause commitment of pluripotent inner cell mass cells to the trophoblast lineage [ 33].
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We scanned all 28,472 approved symbols in the HUGO database as of October 22nd 2009.
Genes may be provided as HUGO approved symbols, or as Entrez or UniProt IDs.
Where available, gene names follow approved symbols by the HUGO Gene Nomenclature Committee and interacting committees, accessed via http://www.genenames.org, and the Zebrafish Model Organism Database (ZFIN), accessed via http://www.zfin.org.org
The gene information we take into consideration includes the approved symbols, approved names, previous symbols and aliases.
IRE structures have been detected in several mRNAs encoding proteins related to iron utilisation (eALAS; mitochondrial aconitase, approved gene symbol ACO2; Drosophila succinate dehydrogenase, gene symbol SDHB), uptake [divalent metal transporter 1 (DMT1), official symbol SLC11A2] and release (ferroportin, gene symbol SLC40A1) (Refs 2, 3, 4, 5, 6, 7, 8).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com