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The absorbance at 680 nm was determined after the sample was appropriately diluted with deionized water to make the absorbance less than 1.0.
Sample (0.05 mL) appropriately diluted with methanol was mixed thoroughly with 0.05 mL FeCl3 solution (4.03 mM in 0.05 M HCl) and incubated for 30 min in a water bath at 37 °C.
To measure DOX contents in the HAssLG nanoparticles, 5 mg of DOX-loaded nanoparticles was dissolved in 10 ml of DMSO/water mixtures (8/2, v/v) and then appropriately diluted with DMSO.
The sample to be measured was appropriately diluted with water and briefly sonicated for 2 min. The measurement of each sample was completed at a scattering angle of 175°.
The digested sample was appropriately diluted with sterile deionized water and filtered with Whatman no.
Test samples were appropriately diluted with PBS containing 2% normal goat serum and added at 200 μL/well.
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Samples were appropriately diluted and stained with 0.1% Acridine Orange before visualizing using an epifluorescent microscope at 100× magnification (oil immersion).
The PCR product was appropriately diluted and supplemented with GeneScan 500 size standard (Applied Biosystems, Foster City, CA, USA) The mixture was then separated on a 50-cm polyacrylamide gel at 15000 V for 45 minutes on an ABI 3730XL capillary sequencer and analyzed with GeneMapper 4.0 software (Applied Biosystems).
Slides were incubated with an appropriately diluted primary antibody before detection with a fluorescein-conjugated secondary antibody.
After incubation with appropriately diluted primary antibodies (see Table 2), membranes were incubated with horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG (Sigma; Dorset, UK) for 1 hour at room temperature and washed.
Appropriately diluted samples were excited with a 480-nm pulsed laser diode (LDH-480) with a repetition rate of 80 MHz.
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