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To detect OPA1 oligomers by BN-PAGE, mitochondria were resuspended in an appropriated volume of Buffer D (1 M 6-aminohexanoic acid, 1.25% V/V digitonin, 50 mM Bis-Tris-HCl [pH 7]) at a final concentration of 10 mg/ml.
Similar(59)
The working solution (10 mg/L) was prepared by diluting the stock solution to appropriate volume.
There is an appropriate volume for every word, in every scene, of every play or script.
Page 1 is a megaphone, and the same piece, run inside the paper and at less imposing length, could have been delivered at a more appropriate volume.
Such citations are abbreviated as AT, followed by the appropriate volume and page numbers.
This has been abbreviated as CSMK, followed by the appropriate volume and page numbers.
Samples were diluted in 0.5 M HCl to the appropriate volume.
After incubation, the cells were washed four times with appropriate volume of complete medium.
The pellet was resuspended in appropriate volume of water by vigorous sonication.
The solution was prepared by accurately adding appropriate volume of each of the prepared standard solution.
For the sorption experiment, an appropriate volume of PHE and PYR dichloromethane stock solution was respectively added to each tube.
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CEO of Professional Science Editing for Scientists @ prosciediting.com