Sentence examples for appropriate volume of sample from inspiring English sources

Then an appropriate volume of sample or standard Pb2+ solution was added and the final volume was made to 5 mL with distilled water.

Appropriate volume of sample and/or standard solution containing acetaminophen in pH 2.2 Mcllvaine buffer solution was injected into the proposed FI system and mixed with the flowing stream of supporting electrolyte (pH 2.2 Mcllvaine buffer solution) at an optimum flow rate of 1 ml min−1.

An appropriate volume of sample or standard solutions were transferred to a 10.0 mL standard flask, then 1.0 mL of 0.30 M sulfuric acid, 1.0 mL of 5.2 × 10−4 M MCP and 1.0 mL of 0.013 M potassium bromide solution were added sequentially and the mixture was then diluted to ca. 8 mL.

Briefly, 100 μL of lysing buffer and an appropriate volume of sample were added to MAb 17E4-precoated wells before incubation with shaking at 37°C for 1 h.

After solubilisation overnight, samples were purified using a 2D Clean Up kit (Bio-Rad, Hercules, CA, USA), resuspended in an appropriate volume of sample buffer, and quantified using the EZQ Protein Quantification Kit (Invitrogen, Carlsbad, CA, USA).

Microemulsions were prepared by mixing appropriate volumes of sample, propan-1-ol and nitric acid aqueous solution, and a stable system was immediately and spontaneously formed.

For the insoluble fractions, pellets obtained after sonication and centrifugation of E. coli cells, were resuspended in an appropriate volume of Laemmli sample buffer.

To the rest of the protein extract, appropriate volume of 5X sample buffer (200 mMTris- Cl pH 6.8, 400 mM DTT, 4% SDS, 0.025% Bromophenol blue, 20% glycerol) was added and boiled for 5 min before loading on SDS-PAGE.

Then collect the remaining cells from each well and pellet by centrifugation at 1,500 rpm for 4 min. Discard the medium and re-suspend the cells directly into the appropriate volume of Laemmli sample buffer to run 10 cells/lane of a gel.

The cells were then pelleted by centrifugation at 1,500 rpm for 4 min, re-suspended directly into an appropriate volume of Laemmli sample buffer sufficient to enable the loading of 10 cells/lane on the gel, lysed by sonication, boiled for 5 min and stored at -20°C.

The protein samples were mixed with appropriate volume of 6 × SDS sample buffer and analyzed using 4 20% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) (Invitrogen).