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Total sample RNA was extracted using commercial kits as appropriate to the sample type; QIAamp Viral RNA Mini spin protocol (Qiagen, Crawley, UK) was used for samples of low cellularity (swabs and BALs), RNeasy Mini kit (Qiagen, Crawley, UK) was used for fresh or frozen tissue samples and RecoverAll™ Total Nucleic Acid Isolation Kit (Applied Biosystems, Warrington, UK) was used for FFPE sections.
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This provided an EPI site- template appropriate to the population sample and with scanner specific image contrast.
Since we included subjects with wide range of age, we created a customized template appropriate to the population samples [ 34, 35].
However, given the large sample size of the interventions group, which was recruited prior to the control group, it was felt that the recruitment method was appropriate to increase the sample size of the control group as much as possible.
This would be especially useful for studies of novel agents (where little is known about the treatment) or for rare disorders, where it is appropriate to minimise the sample size.
Therefore, we should choose analytical methods that are appropriate to the sequencing coverage and sample size in order to use haplotype information in sequencing reads.
A relatively small sample of suitable general practitioners rather than a random sample was appropriate to the needs of this exploratory study.
These sizes were appropriate to sample the small species of algae and invertebrates that characterized the shore and did not result in different estimates of either mean abundances or spatial and temporal variances of species [50], [51].
For the WWT study, LOD by dry weight varied between laboratories, so the value appropriate to each sample was used and then converted to a LOD by wet weight by multiplying it by the dry weight fraction for each sample.
To detect previously undiscovered associations of common genetic variants with reading and language skills, it is therefore appropriate to sample broad ranges of the trait distributions in study datasets, while screening over the entire genome.
In general, it is appropriate to harvest samples at entry into the study, as well as 2 weeks and 4 weeks after treatment.
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