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The appropriate samples were supplemented with 1 mM IPTG (final concentration) and all samples incubated ON at 30°C with rigorous agitation.
Appropriate samples were combined and sonicated on ice.
Because the appropriate samples were not kept, it will not be possible to exclude this possibility.
A prion protein gene analysis could not be performed because appropriate samples were lacking.
Labeled sample DNA was purified by size exclusion on YM-30 filters (Eppendorf) and appropriate samples were combined.
Where appropriate, samples were analyzed by using the Student's two-tailed t-test or ANOVA analysis of variance) by SPSS software.
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In NYS, all specimens from rabies-negative animals of species susceptible to the transmissible spongiform encephalopathies (e.g.Scrapie, CWD, BSE) will automatically be forwarded for testing when appropriate samples are submitted.
For the rhinocerebral form the appropriate samples are as follows: sinus aspirates, tissue specimens from the affected area, and also scraping of the nasal mucosa.
Convergence and appropriate sampling were confirmed examining the standard deviation of the split frequencies between the two simultaneous runs and the Potential Scale Reduction Factor (PSRF) diagnostic.
Both convergence and appropriate sampling were confirmed by examining the standard deviation of the split frequencies between the two simultaneous runs and the Potential Scale Reduction Factor (PSRF) diagnostic.
The appropriated samples were tested for cytomegalovirus, varicella zona virus, mumps virus and enteroviruses with specific culture, and serological and molecular biological techniques.
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