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The PCR product was digested by the appropriate restricted enzymes, then purified and subcloned into the pET28a vector.
The double stranded oligonucleotides were further modified with appropriate restricted endonucleases and cloned into an adenoviral shuttle vector, pAdTrack-CMV (Department of Biological Chemistry, School of Medicine, Fudan University, Shanghai, China).
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And we need to find ways to come up with appropriate guidelines and regulations to, where appropriate, restrict the use of this technology in a situation where it's ill-suited.
In the absence of the proper signal from the appropriate restricting molecule, the developing T cell will fail to become a mature CD8+ T cell.
Note: the School of Medicine encourages the Department to charge the salary over the cap to the operating budget in event there is not an appropriate donor restricted fund.
Director of Allied Health (Rural/Regional) Limited access to appropriate technology restricted opportunities for AHPs to engage in workplace learning.
Nine of 12 shell-lesioned rats were determined to have appropriate lesions, restricted to the shell subregion and not overlapping with any other area (Fig. 3Aand B), with the lesion focused on the caudal shell.
Eight of 12 core-lesioned rats were determined to have appropriate lesions, restricted to the core area of the NAc and not overlapping with the shell region (Fig. 2Aand B).
This dictum seems most appropriate when restricted to the organ predilections contingent to the seed and soil hypothesis [ 32, 44], and to the anatomic pathways of regional metastasis [ 45, 46].
Eight of 10 NAc core lesion rats were determined to have appropriate lesions, restricted to the core area of the NAc and not overlapping with the shell region (Fig. 2).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com