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Artificial chromosome based systems are composed of AC engineered to harbor and express gene(s) of interest and an appropriate recombination system for 'custom' engineering of ACs.
Next generation sequencing of crop genomes could reveal pseudo recognition sites, which would allow choosing the most appropriate recombination system by crop[ 148].
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Site-specific recombination systems are potent genome modifiers, and they can be used to integrate DNA into loci that contain an appropriate recombination signal sequence, either naturally occurring or suitably pre-engineered.
Results: Here we analyze the effects of recombined and knotted plasmids in E. coli using the Hin site-specific recombination system.
Cre/loxP is a widely used site-specific DNA recombination system derived from bacteriophage P1.
We validate the functionality and specificity of this unique recombination system, in which the recombinase and recombination directionality factor (RDF) are encoded in separate genomes.
The FLP recombination system, derived from the 2μ plasmid of Saccharomyces cerevisiae, mediates site-specific intramolecular (excision or inversion) and intermolecular (integration) recombination between FRT sites.
This problem can be circumvented using a Cre/loxP recombination system.
En Passant Mutagenesis: A Two Step Markerless Red Recombination System / Nikolaus Osterrieder.
This new genetic tool relies on the transposon γδ site-specific recombination system.
Here we propose a gas recombination system design that operates independent of pump orientation.
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