Although we focused on a one-step RT-PCR to decrease handling and total assay time, the three real-time RT-PCR assays can also be performed as two-step RT-PCR, including a separate cDNA synthesis step followed by PCR, and then finally using appropriate ready-to-use master mixes and the same cycling conditions, omitting the RT step.
Salle is known for mixing modes of representation and appropriated ready-made motifs in a single canvas, suggesting but defying any legible narrative.
Like many artists of the 1980s, Gonzalez-Torres used the postmodern strategy of appropriating ready-made motifs and objects to create his art, thereby challenging the idea of the unique art object that was so much a hallmark of Modernism.
Other meats (chicken, pork loin, beef or short ribs) were cooked in the kitchen and tossed with appropriate sauces, ready to be made into lettuce packages at the table.
As electronic health records evolve, this historical approach is disappearing and new efforts by EIP staff are required to gain appropriate and ready access to electronic records and new skills are needed to use them effectively.
Anaesthetic time was defined as from the conclusion of check-in to the time when the patient was positioned (in the supine or prone position as appropriate) and ready for draping and surgical preparations to begin.
Show up at the appropriate time, ready to go.
Have appropriate music ready when you get home.
You can cross to the USA (Buffalo) or to the Canadian side via a bridge - make sure to have your passport or appropriate ID ready.
Communications are generated, as appropriate, as ready-to-send emails or ready-to-print letters; ePOCS correspondence is entirely electronic for patients able and willing to provide a contact email address.
To prepare due communications, administrators click the red-highlighted buttons; the required communications are automatically populated with the relevant patients' details and generated, as appropriate, as ready-to-send Emails or ready-to-print letters.
