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Then, immunoblotting was performed with appropriate primary antibodies at 4 °C overnight.
After blocking with 5% BSA, the cells were stained with the appropriate primary antibodies at 4 °C overnight.
Cells were then blocked with 5% Bovine Serum Albumin (BSA, Jackson Immuno Research, West Grove, PA, USA) before sequential incubation with appropriate primary and secondary antibodies (Table S1).
Europe said Greece needs to keep running "appropriate primary surpluses... in line with the targets agreed to," but added that there's "flexibility" here.
Samples were then incubated with the appropriate primary antibody or with fluorescein isothiocyanate-conjugated Isolectin B4 (Sigma) overnight at 4 °C.
The membranes were blocked with 5% BSA (bovine serum albumin) for 0.5 h, followed by incubation with appropriate primary antibodies overnight at 4 °C.
Despite considerable interest in the subject, there is still no consensus on the most appropriate primary therapeutic modality.
Urban minority children are at risk for poor asthma outcomes and may not receive appropriate primary or subspecialty care.
The focus on primary care is appropriate; primary care is the first line of defense and yields large dividends.
For each input, this fuzzy decision-making system must be able to select the appropriate primary diagnosis.
The blocked blot was exposed to the appropriate primary antibody in blocking buffer with constant mixing.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com