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While F2365 shows at least 20 authentic mutations resulting in premature stop codons and demonstrates some atypical invasion characteristics [ 53], the genome for this strain was included in our analyses to provide us with increased power for our analyses and an appropriate number of sequences to perform the lineage specific analyses for positive selection.
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However, with the enormous sequencing output from current sequencing platforms, generating a sufficient number of amplicons across an appropriate number of samples to avoid unreasonable sequencing depth and cost is prohibitive.
The cDNA was amplified using the appropriate number of PCR cycles to produce sequencing libraries, which were subsequently subjected to the proprietary Solexa sequencing-by-synthesis method using the Illumina Genome Analyzer (SanDiego, CA, USA).
The cDNAs were amplified using the appropriate number of PCR cycles to produce sequencing libraries, which were subsequently subjected to the proprietary Solexa sequencing-by-synthesis method using the Illumina Genome Analyzer (SanDiego, CA, USA) at the Beijing Genomics Institute (BGI, Shenzhen, China).
RP can be a good target for massively parallel sequencing as an appropriate number of candidate genes are known for RP.
o Two "Update tree" options allow redrawing the similarity tree by setting the appropriate number of top-scoring BLAST hits or using a user-defined sequence selection.
Determining appropriate number of clusters.
Finding the appropriate number of copies is important in DTNs.
An appropriate number of layers is sufficient for this task.
The appropriate number of patches is determined automatically.
Determining the appropriate number of insurers poses trade-offs.
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