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For infections, bacteria were resuspended in sterile water and diluted to an appropriate multiplicity of infection (MOI) based on optical density (OD) at 600 nm.
Cells were transduced by lentiviruses with an appropriate multiplicity of infection in complete growth medium supplemented with polybrene (Sigma Aldrich).
Cells were then infected with Ad-ChM1 or Ad-LacZ as a control, at an appropriate multiplicity of infection (MOI) and were further cultured.
Similar(57)
Despite the multiplicity of appropriate responses to the questions, however, the reasons for asking them basically reduce to two, and they hold not only for Chinese writings, but all foreign philosophical texts.
One suggestion has centred around the idea that there can be a multiplicity of appropriate or correct divergent interpretations of one and the same artwork which cannot be reduced to one underlying interpretation or ranked in relation to each other (e.g., Margolis 1991; Goldman 1990).
When appropriate, we corrected for multiplicity of statistical tests using standard procedures [54], [55].
Tenocytes were infected with the appropriate adenoviral vector at a multiplicity of infection of 35 using Xtremegene HP (Roche Diagnostics Ltd ,Burgess Hill, UK) following the manufacturer's instructions.
CB CD34+ cells were cultured overnight as previously described and then underwent two rounds of transduction with the appropriate lentiviral supernatant at a multiplicity of infection of 100.
"Rather, it would be appropriate to reflect (completely independently of the multiplicity of the historical material) on that which holds valid by necessity, that is, the formal presupposition of each value-judgment claiming more than individual validity.
M. smegmatis mc2155 cells were infected as described above with the appropriate amount of phage to obtain a multiplicity of infection (moi) of 100, 10, 1 or 0.1.
The virus was diluted at the appropriate concentration in serum-free MEM (reaching a multiplicity of infection of around 500; this MOI was necessary in order to observe enough events that could be used for tomographic reconstruction).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com