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Washed cells were then incubated with the appropriate labeled secondary antibody and resuspended in PBS.
Tissue whole mounts were washed three times with DPBS and incubated for 1 h at 37°C with the appropriate labeled secondary antibodies (goat anti-mouse IgG or goat anti-rabbit IgG coupled to Alexa 488 or Alexa 594).
Control experiments included incubation of sections or cells with non-immune isotypic control antibodies or the omission of primary antibodies followed by the appropriate labeled secondary antibodies.
The primary antibodies were then detected using the appropriate labeled Streptavidin-Biotin (LSAB) kit (Maixin Biotechnology, Fuzhou, China) according to the manufacturer's instructions.
PCR reactions were performed in duplicate in 20 μl volume and included approximately 30-50 ngenomicic DNA, 1.5 U Taq polymerase, 12.5 pmol of each appropriate labeled PCR primer, MgCl2 concentration as appropriate to the PCR primer set pair (Additional file 1, Table S1) and 0.2 mM of each of dATP, dCTP, dGTP, and 1 60 ratio of dTTP: dUTP in distilled H2O.
Don't rinse any of the chemicals down the sink; put into an appropriate, labeled waste container.
Similar(54)
But "year of insanity" seems like a more appropriate label for 1973.
When the participants found out whether their choice was right or wrong, they were also shown the appropriate label.
And they said that their study, while hugely controversial, was relatively simple: figuring out the appropriate labels to place on the behaviors in the individual cases.
In both cases, identity is construed as a recognisable object, a specific something with a given content that can be tagged with an appropriate label.
After Thierry Henry's sleight of hand dumped the Irish Republic out of the World Cup last year, there was less unanimity about an appropriate label for the event: Handgate, Henrygate or Thierry-gate?
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com