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The E. coli expression system used here is probably not appropriate for the expression of TomLOXD.
100 μg ml−1 ampicillin or 20 μg ml−1 gentamicin, were added when appropriate; for the expression of the endogenous E. coli cytochromes no antibiotics were used.
In plant expression systems, leaves and seeds have both advantages and disadvantages, and both seem appropriate for the expression of all targeted proteins.
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Protein extracts were prepared from HLF cells treated with 1 μM ACR alone, 5 μM LY294002 alone, or a combination of these agents for 12 hours because this treatment time was appropriate for evaluating the expression levels of phosphorylated extracellular signal-regulated kinase (p-ERK), phosphorylated Akt (p-Akt), and phosphorylated RXRα (p-RXRα) proteins [ 25, 29, 30].
The results indicate that FPV recombinants can express correctly the E6 and E7 oncoproteins, and they should represent appropriate vectors for the expression of these oncoproteins in human cells.
The authors also showed that the yeast P. pastoris is an appropriate host for the expression of the S. bovis α-amylase-LysMAcmA fusion protein.
It was one of the first examples illustrating that insect cells were appropriate hosts for the expression of plant genes: ABP1 was correctly processed and glycosylated in the endo-membrane system of insect cells.
No AI-2 induced genes other than this transporter have been found, indicating that in this organism AI-2 may not serve as a quorum sensing signal or the appropriate cultivation conditions for the expression of its activity were not met.
Therefore, we considered this 70-mer array, designed from the N. crassa genome sequence, to be appropriate for the analyses of expression of N. intermedia isolates, especially when analyzing differences in relative expression between strains of N. intermedia and not between N. intermedia and N. crassa[ 65, 66].
We assume that the expression values have already been background corrected, normalized and, perhaps, filtered in a way appropriate for the microarray or expression platform.
The proportions of UE annotated in each category were generally similar to those obtained in Arabidopsis (Additional file 4), suggesting that the E.spp sequencing sets are appropriate for the analysis of gene expression on a broad range of functional categories.
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