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The choice of an appropriate expression system remains empirical, largely depending on the biochemical and biological properties of the target proteins (Bernaudat et al., 2011).
To conclude, L. lactis appears to be an appropriate expression system for Arabidopsis membrane proteins.
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Choosing the appropriate expression systems is important for the enzyme production rate, and bacteria, filamentous fungi, and yeasts have been used to express recombinant enzymes.
This model is supported by studies in which p53 levels may be artificially controlled with the appropriate expression systems [9], [10].
For the establishment of such properties, the employment of appropriate expression systems permitting the synthesis of larger quantities of the ORs is required.
All these approaches employ a similar principle for the construction of the library: cDNA is enzymatically converted to short inverted repeats separated by a linker-sequence and transcribed into shRNAs by appropriate expression systems.
For the latter, target genes are cloned after being amplified from metagenomic DNA by using polymerase chain reaction with conserved sequences as primers; alternatively, they may be directly discovered from metagenome sequence databases using bioinformatics tools, subsequently amplified, and cloned in the appropriate expression systems.
The aim of this work was developing an appropriate heterologous expression system for VanYn characterization to help deciphering its role in glycopeptide resistant cells.
In order to investigate VanYn role in glycopeptide resistance in the producer actinomycete an appropriate host-vector expression system is required.
Taken together, our data provides evidences that rArtinM will be a useful tool for future biomedical studies and that E. coli expression system is appropriate to produce large quantities of functional ArtinM for industrial purposes.
Choosing an expression system capable of generating an appropriate product PTM profile remains one of the most crucial decisions a drug developer must make.
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