Sentence examples for appropriate confluence from inspiring English sources

In this framework, one can unify costs of model development, external data acquisition, and those of the time value of predictions; this facilitates the development of strategies that derive synergy from the appropriate confluence of model development and external data acquisition.

The CRC lines were cultured under standard conditions with 10% (v/v) bovine serum in the medium, and split as appropriate before confluence was reached.

Cells were plated in the chamber and when they reached the appropriate density (90% confluence, to 5 days post-confluence), the growth medium was replaced with Calcium-free DMEM supplemented with 5 mg/ml Lucifer yellow (7).

When cells reach about 80% of confluence in appropriate culture dishes, cells were pre-starved using DMEM supplemented with 0.5% FBS for 2 hrs and then pretreated with gAd (2.5 ug/ml) for 1 hr.

Cells were seeded at the appropriate concentrations to prevent confluence throughout the experiment.

Each cell line was grown to complete confluence in appropriate media.

Transfected cells were grown for 24 48 h in 8 well Nunc Labtek chambered coverglasses coated with poly-L-lysine (Fisher, Loughborough, U.K ., including tetracycline pretreatment as appropriate, to approximately 80% confluence.

Transwell filters of 8-mm thickness (Corning Incorporated, Acton, MA, USA) were coated with 1% gelatin, and endothelial H5V cells (previously pre-activated by 12 h exposure to TNF- α to induce the expression of surface antigens and adhesion molecules required for the appropriate extravasation) were plated to confluence on them for 24 h.

Cells were grown in appropriate media until they reach 60% confluence.

24-well plates were seeded with VK2 or HeLa human epithelial cells in appropriate media and grown to >90% confluence.

Cells were cultured for 12 15 days post-confluence, which represents an appropriate period to reach full differentiation and study lipid and lipoprotein metabolism as well as oxidative stress and inflammation [ 16].