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A pianist, sometimes accompanying a singer, plays appropriate cocktail music in the lounge, a dignified room with many overstuffed sofas and chairs.
Appropriate cocktail party repartee among a circle of social wits, yes.
Cells were washed and then stained for 30 minutes with the appropriate cocktail of the following antibodies from BD Biosciences: CD4-PerCP-Cy5.5, CD8-APC, Vβ8 FITC, Vα2-PE, CD69-PerCP-Cy5.5, CD44-FITC, CD25-PE, CD62L-APC and TCRCα-APC.
The primer extension reaction was performed in a volume of 10 µl, containing 3 µl of PCR product, 2.5 µl of SNaPshot Multiplex Ready Reaction mix, and the appropriate cocktail of PAGE-purified extension primers (IDT Supporting Information Tablee S6B).
Cell suspensions were blocked with 2.4G2, anti-FCγR washed, resuspended in FACS buffer (PBS, 2 mM EDTA, 2% FBS, 0.2% NaN3) containing the appropriate cocktail of antibodies and incubated on ice for 20 min. For intracellular cytokines detection, Fix and Perm® kit (Invitrogen) was used according to manufacturer's instructions.
Men should look trendy but tuxedos are not appropriate cocktail attire.
Similar(54)
After an appropriate cocktail-length wait, they arrive in two neat stacks.
Most of the guests wore nametags, and many sipped historically appropriate cocktails, called gin daisies.
(Only recommended for white wine and appropriate cocktails. I'm an advocate of prudence, not barbarianism).
There's an entire Tumblr blog, Proof Reading (proofreadingbooks.tumblr.com), dedicated to matching books with appropriate cocktails.
The following day, sections were washed three times with TBS and were then incubated with appropriate cocktails of secondary antibodies (i.e. biotinylated donkey or goat anti-rabbit; 1 400 and Cy3-conjugated goat anti-mouse or donkey anti-goat, 1 200 Dianova) for 60 min at room temperature.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com