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The substrate specificities among these MerB enzymes show the necessity for selecting the appropriate bacteria strains or MerB enzymes to apply them in bioremediation engineering for cleaning up specific organomercurial contaminations.
On day 11, 10 µl of 0.0002 ABS600 of exponentially growing cultures of appropriate bacteria were added to each well and incubated at 25°C until the indicated time points.
Overnight cultures of the appropriate bacteria were inoculated 1 50 into DMEM and primed as described previously (Collington et al., 1998).
To prime bacteria for infection, DMEM was inoculated with overnight cultures at a dilution of 1 50 of the appropriate bacteria as previously described (Collington et al., 1998).
The knowledge of the specific enzymes and mechanisms involved in these processes could be helpful in the selection of appropriate bacteria to be used in bioremediation programs.
The authors finally decide to perform the proteome analyses on worms cultivated on LB media with the appropriate bacteria due to the unambiguous negative influence on the worms' life span upon S. aureus infection.
Similar(53)
Such an approach might be especially appropriate for bacteria where homologous recombination among repeats appears to play a major role in genome rearrangement.
After appropriate dilutions, bacteria were washed twice with phosphate-buffered saline and mice were fed 50 µl of the solution containing 109 CFU with a pipette to the back of the oropharynx after fasting for 16 h.
Once a sample arrives at the appropriate laboratory, the bacteria must be cultured to provide enough DNA to test, but an Air Force research laboratory recently developed growth media -- essentially bacteria food -- tailored to accelerate the growth of anthrax bacteria, but not other varieties.
It showed that appropriate numbers of bacteria were treated.
Dr. Fan and his colleagues hypothesized that the lack of appropriate nitrogen fixing bacteria in the soil was the cause of the failed introduction.
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