Sentence examples for appropriate amount of template from inspiring English sources

The appropriate amount of template cDNA and number of PCR cycles were determined for each gene to ensure that amplification occurred in the linear range and that amplified products were quantified accurately.

Following a standard protocol, PCR reactions contained appropriate amounts of template cDNA (2 to 10 ng), 50 mM KCl, 20 mM Tris HCl (pH 8.4), 1.8 mM MgCl2, 200 μM dNTPs, 1 unit Taq polymerase, and 0.4 μM of each primer (detailed primer information is given in Additional file 7) in a total volume of 25 μl.

The PCR reaction was set up in 50 μL reaction volume: 5 μL 10X pfu turbox buffer; 1 μL 10μM dNTP; 2.5 μL 10 uM forward and reverse primer; 1 μL pfu turbo cx polymerase (Agilent); appropriate amount of DNA template and water added up to 50 μL.

An appropriate amount of DNA template was used to generate between 1 and 5 mutations per 1 kb.

The appropriate amount of first-strand cDNA template was mixed with specific TaqMan primers (Applied Biosystems, Foster City, CA, USA), water and PCR Master Mix (Applied Biosystems), and quantitative RT-PCR was conducted using a 7900HT Fast Real-Time PCR system (Applied Biosystems).

The growth of vertically aligned carbon nanotubes has been successfully reported using a porous template and the appropriate amount of catalyst such that the pores can physically hold the nanotubes in the vertical direction and the bundles of nanotubes can support each other during growth.

Real-time quantitative RT-PCR was performed in a ChromoTM 4 (MJ Research, USA) using the SYBR Green RT-PCR KiTakaraara, China) and 10 μL reaction volumes, which contained 5 μL of SYBR Green PCR mix, 0.2 μM of each forward and reverse primer, 1 μL of diluted cDNA template, and the appropriate amount of sterile ddH2O to achieve the desired reaction volume.

After obtaining the first double-stranded cDNA, the appropriate amount of amplified single-stranded cDNA was used as a template for biotin labelling using the FL-Ovation™ cDNA Biotin Module V2.

"This is an appropriate amount of time.

Worried parents have taken to wandering in a maze of intricacies of what is and isn't appropriate -- appropriate language; appropriate amount of violence, sex, fear, diversity; appropriate amount of stimulation and lack thereof; appropriate amount of play of the imagination; appropriate amount of good and bad news about life.

After an appropriate amount of foreplay, he slipped on his condom, entered her, and, after an appropriate amount of time, ejaculated.