Sentence examples for appropriate amount of cells from inspiring English sources

Then the appropriate amount of cells (42 168 mg dry weight harvested by centrifuging at 5000 g for 15 min) was transferred to 100 ml of nitrogen free medium for curdlan production.

Two-step culture technique was employed and the appropriate amount of cells grown in YP medium was transferred to nitrogen free fermentation medium at different pH ranging between 5.0, 5.5, 6.0, 6.5 and 7.0.

An appropriate amount of cells grown at 30°C was harvested (1100 rpm, 8 min) and washed twice with PBS.

An appropriate amount of cells was harvested, washed with PBS, spun (350 rpm, 3 min, RT) on 0.05% polylysine-coated coverslips and fixed with 4% paraformaldehyde for 30 min. Cells were further washed with wash buffer (PBS with 0.3% TritonX-100 and 0.05% saponine) and blocked for 1 h in blocking buffer (wash buffer with 5% BSA).

Saturated 3 ml cultures were counted, and an appropriate amount of cells was removed from each culture to inoculate 300 ml of BMW at 1 × 10 cells/ml.

An appropriate amount of cells of the intermediate culture (to obtain an OD600 of 0.2 in 5 mL) was pelleted by centrifugation at 845  g for 5 minutes, and washed once by resuspending in 750 μL synthetic glycerol medium.

On the basis of the Sav FBS determination, add the appropriate amount of cell lysate or CFE to an HPLC vial with a magnetic stir bar.

The pellets were then re-suspended in the appropriate amount of cell lysis buffer (1X PBS+1% Triton-X 100) and incubated on ice for 30 min. Following the incubation, the homogenates were centrifuged at 14,000 rpm for 10 min at 4°C and the supernatants were transferred to 1.5 ml Eppendorf tubes.

The detection of an effect on the fraction of G2/M-positive cells after incubation with TMZ might require an appropriate amount of cell division, which is only reached after 48 h.

After centrifugation for 5 min at 9000 × g, an appropriate amount of cell lysates was added to the reaction mixture, containing 100 μM NADPH in a final volume of 200  μl PBS (pH 7.4).

XI assays were performed using buffer containing 100 mM Tris-HCl, pH 7.5, 10 mM MgCl2, 0.15 mM NADH, 2 U sorbitol dehydrogenase (Roche, Mannheim, Germany), and an appropriate amount of cell lysate.