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Moreover, we were able to demonstrate a proof-of-concept for this protein concerning proteomic approaches with subsequent validation by protein-biochemical approaches.
Purification of the analytes is often achieved by using sophisticated sample cleanup approaches with subsequent separation by liquid chromatography and detection using triple-quadrupole analyzers coupled via an electrospray ionization (ESI) interface.
Identification of these processes by the "omics" approaches, with subsequent evaluation in a systems biology framework, and linking the findings back to traditional end points will help develop diagnostic tools specific to BRD.
To determine the role for PEA3 in the regulation of Fgf-10 expression, we used both RNAi-mediated knockdown and recombinant overexpression approaches, with subsequent real-time RT-PCR analysis.
Notably, miR146a was also found to be down-regulated in hippocampal neurons after Aß42 treatment in the Schonrock study (Schonrock et al, 2010)—albeit not in the APP mouse model indicating the need to link screening approaches with subsequent mechanistic studies.
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Herein, we report the structural engineering of V2O5/graphene with different morphologies by a solvothermal approach with subsequent annealing in air.
To explore the power of computer science in supporting experimentally based process development, we applied a combined force field approach with subsequent Density Functional Theory (DFT) calculations.
Li3VO4@LiVO2 composite with a specific architecture of Li3VO4 crystals homogeneously embraced by LiVO2 matrix is fabricated via an intermediate solution approach with subsequent sintering.
(a) 42% thresholding, (b) EMGMM, (c) GMRF, (d) statistical approach acting on MIP [40], (e) fuzzy locally adaptive Bayesian approach [38], and (f) maximum a posteriori GMRF approach with subsequent deconvolution [39].
We extracted total genomic DNA, and employed a degenerate primer approach with subsequent cloning to obtain fungal PKS sequences.
The use of a multiscale approach, with subsequent atomistic simulations, in part addresses this limitation.
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