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Using biochemical, genomic and in vivo approaches, we identify PARP3 as an important transcriptional regulator acting early in the development of sensory placodes and in the specification of neural crest cells of zebrafish embryos.
Here, using bioinformatic and biochemical approaches, we identify the AMPA receptor GluR2L and GluR4 subunits as novel physiological JNK substrates in vitro, in heterologous cells and in neurons.
By using drug inhibition and gene inactivation approaches, we identify eNOS dysfunction as the primary cause of ROS increase in bar mutant and UBIAD1-silenced human ECs.
Using RNAi and chemical genetic approaches, we identify DOT1L as a histone methyltransferase required for the mitotic-associated increases in H3K79me2.
This module contains our putative candidate gene and through a series of bioinformatics approaches we identify a potential mechanism for this QTL, through altered macrophage gene expression.
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Using these approaches we identified 33 IE genes, 22 DE genes and 36 L viral genes.
What mediates the translational regulation function of RA? Combining electrophysiological, biochemical and ultrastructural approaches, we identified a novel role of the RA-receptor RAR& 945; in translational regulation.
Based on multiple complementary approaches, we identified CG31665 as a novel gene causing dilated cardiomyopathy.
Using these approaches we identified 3189 promoter binding sites representing 12.8% of promoters analyzed.
Finally, using RNA interference and chromatin immunoprecipitation approaches, we identified a transcriptional cofactor participating in GR-activated DUSP1 gene transcription.
Using these screening approaches, we identified a total of 36 deletion strains that suppressed BRCA1 lethality (Table S1).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com