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Using different fluorescence fluctuation approaches, we established that paxillin-EGFP is dynamic on many timescales within the cell, ranging from milliseconds to seconds.
In order to establish conditions for the application of pePNA molecules for antisense approaches we established an assay for translation blocking.
With these screening approaches we established a set of tagged master cell lines that stably express the respective reporter gene(s).
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Using Fas siRNA and overexpression approaches we establish that Fas is not a pro-apoptotic factor but a contributor to cell protection in HePC-apoptosis-sensitive cells.
With these approaches we establish here an overall framework of information that will facilitate not only further research regarding the functional characterisation of Jmjd6 but also of other members within the DSBH-domain containing superfamily of proteins.
In this study, using a combination of biophysical, biochemical, structural, and cell biological approaches, we establish that self-assembly of SAS-6 homodimers is at the root of the universal 9-fold symmetry of the cartwheel and thus of centrioles.
Using this approach, we established efficient incorporation of non-canonical amino acids within HIV-1 Env in mammalian cells.
To validate our approach, we established a conformational map of the Vibrio vulnificus add adenine riboswitch that reveals five classes of structures.
As a result of this pioneering approach, we established the combinatorial expression of the sets of genes that could dictate the functional specializations of MRGPRD+ neurons and C-LTMRs.
Using fluorescence in situ hybridisation (FISH) approach, we established its localisation on the chromosome 11q23.2.
Following a similar approach, we established an in vivo tumor xenograft model based on HeLa-GFP-tm cells.
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