These findings indicate that the proteomic approach utilized here was sufficient to identify surface-exposed peptides from membrane proteins.
The approach utilized here involves RNA isolation from testes followed by cDNA synthesis for use as a template in RQ-PCR.
Our findings help to elucidate the role of A-to-I RNA editing in the regulation of tissue-specific development and function, and the approach utilized here can be broadened to study other types of tissue-specific substitution editing.
The integrative approach utilized here where two clinical subtypes (primary vs. metastases) were compared on both genome-wide copy number and expression levels is a productive methodology for identifying metastasis-relevant genes, as reflected by our ability to define a short list of candidates that included MET receptor tyrosinase kinase and BIRC5.
Moreover, the TO2 approach utilized here is applicable to many other areas of neuroscience.
The combined metabolomics and transcriptomic approach utilized here has provided some insight into the observed inducible nature of tanshinone production in hairy root cultures of S. miltiorrhiza.
However, the fluorescence approach utilized here measures the kinetics of ERAD substrate exposure to the cytosol continuously at a time resolution higher than that achievable by other methods.
Finally, the qPCR approach utilized here, using gDNA to normalize expression estimates, could provide more reliable results than the typical alternative of normalizing to expression of a single reference gene in any instance where relative expression estimates are needed.
The microarray approach utilized here has alleviated many of the past difficulties plaguing the molluscan biomineralization discipline, however, this method and its' outcomes is in no way seen as a standalone conclusion.
The computational approach utilized here, assessing size polymorphism at repeat loci, presents an instantaneous computational view of strand slippage acting in the genome because the presence of indel polymorphisms reflects few, relatively recent mutational events.
