Exact(4)
Lately, major attention has been paid to biotechnological approaches, using cell extracts or whole-cell systems for the production of -nootkatone.
Although blood is recognized as a highly important source of disease-related biomarkers, the complexity and dynamic range of protein abundance in body fluids has hampered proteomic biomarker discovery and alternative approaches using cell models may be more successful.
Therefore, the results of GVL appear to correlate better with clinical outcome than the traditional classification approaches using cell morphology and IPSS score.
It is the first to examine the subcellular localization of native TTP protein in freshly isolated cells, since all previous studies relied on prolonged cell culture or transfection/overexpression approaches using cell lines [ 20- 24, 25, 25].
Similar(4)
These results suggest that the application of GWA approaches using cell-based model systems, when combined with complementary functional validation, can provide insights into mechanisms responsible for variation in cytidine analogue response.
In vitro approaches use cell seeding, spheroid formation or cell sheet technologies.
These approaches use cell lysates as a source of T. brucei kinase protein and specifically enrich for kinases using different matrices containing immobilized kinase inhibitors or other kinase-binding compounds.
The previously discussed approaches use cell lines mixed with primary cells but to fully exploit the potential of co-culture as a means of achieving a more organotypic model, primary hepatocytes would ideally to be co-cultured with primary cells from the same organ.
Related(2)
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