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However, most downstream processing methods mainly rely on expensive chromatographic approaches to purify the fusion proteins, which lower the overall protein yield substantially (Azevedo et al. 2009).
In fact, monolithic supports have emerged as interesting approaches to purify pDNA due to their excellent mass transfer properties and high binding capacity for large biomolecules.
In addition, approaches to purify recombinant AChEs to a degree that is suitable for analytical applications will be elucidated as well as the various attempts that have been undertaken to increase the sensitivity of AChE to specified organophosphates and carbamates using side-directed mutagenesis and employing the enzyme in different assay formats.
However, studies designed to dissect the anti‐apoptotic role of Hhs have been hindered by the lack of simple approaches to purify large quantities of recombinant ligands in the average laboratory setting because of the natural modifications of these proteins with palmitic acid and cholesterol.
Major approaches to purify ubiquitin and ubiquitin-like proteins include the use of epitope-tagged forms of these small protein modifiers, specific traps, and specific antibodies that enable the purification of endogenous ubiquitin and ubiquitin-like proteins.
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Forward looking approaches to purifying and delivering DNA are reported, including affinity chromatography and nasal inhalation.
With a growing cultural emphasis on sustainability, an alternative environmentally friendly approach to purify water on a micro scale is via the solar still (solar distillation).
We reasoned from the calculated pKa of [18F]AV1451 (Fig. 4), as well as a literature report of the pKa of γ-carboline (Angulo et al. 1997), that the use of a basic medium was the optimal approach to purify this radiotracer.
We used this approach to purify gemin5 and perform subsequent cap-affinity chromatography in the presence of specific (m7GpppG) or nonspecific (GpppG) cap analog competitors (Figure 5A).
We recently exploited an efficient approach to purify gold nanoparticles in the 20 250 nm size range by continuous density gradient centrifugation.
Our previous work [10] showed that GST-FLAG-P7 accumulated within inclusion bodies that could be selectively harvested, and we applied this approach to purify GST-FLAG-SH.
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