This scoring method followed similar approaches previously demonstrated for reduced risk of chronic diseases in this cohort (Cooper et al., 2012; Jeurnink et al., 2012), and reflected the minimum 2 weeks needed for a person to exhaust the variety of their food repertoire (Drewnowski, Henderson, Driscoll, & Rolls, 1997).
While a variety of analysis approaches can be conceived for detecting statistically significant differences in allelic frequency, we chose to implement an approach previously demonstrated effective at identifying the correct genetic loci in other pooling-based studies in complex diseases [8].
Printed glycan array technology (a glycan-based discovery approach) previously demonstrated that naturally occurring anti-glycan antibodies (AGA) in plasma of ovarian cancer patients exhibited specificities towards synthetic P1 trisaccharide.
In different cell systems, and using different mechanistic approaches, we previously demonstrated that an impaired RARα signalling, due to derangement/loss of RARα itself, confers an exacerbated repressed chromatin state, marked by repressive epigenetic changes at several RA-responsive genes downstream of RARα [7] [9].
Two quite similar approaches were previously demonstrated in familial hypercholesterolemia [ 28] and autism cases [ 29].
In accordance with this approach we previously demonstrated that in MOH patients with low medical needs and migraine as the primary headache type advice and education were as effective as structured inpatient and outpatient detoxification programmes in achieving withdrawal of the overused medication/s [24].
Using this approach, we previously demonstrated that serum levels of purine metabolites correlated with on-aspirin platelet reactivity.
Using a loss-of-function approach we previously demonstrated that Nanog is necessary for the formation of embryonic and induced pluripotency [ 11].
Using this multimarker approach, we previously demonstrated that patients at later clinical disease stages have significantly greater numbers of CTCs than those at earlier stages [ 20].
Using this approach, we previously demonstrated patterns of positive BOLD changes that were mostly concordant with the presumed source of the IED as inferred from electro-clinical data [Salek-Haddadi et al., 2006].
