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These include, but are not limited to, approaches of sequence alignment, variant discovery, expression analysis and de novo assembly.
Studies about the role of SD in Vitis vinifera and other plant genome evolution have followed classical assembly-based approaches of sequence alignment and comparison [ 4, 19- 22], thus ignoring the impact and contribution of highly similar SDs.
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In future, novel approaches of sequencing, such as third and fourth generation sequencing can alter the workflow, cost, analysis, and interpretation of genomics associated with PCC/PGL.
Relatively few studies have investigated (with success) selection approaches of sequences homologous to those found in the genome sequences of fungal model species to develop PCR primer-based markers in non-model-ones [9], [26], [27], [28].
A collective approach of sequence analysis, phylogenetic tree and in silico prediction of amyloidogenecity using bioinformatics tools have been used to correlate the observed species-specific variations in IAPP sequences with the amyloid forming propensity.
Using such a yardstick will call into question the very approach of sequence analyses.
We have developed and applied a two-dimensional approach of sequence analysis, called Hydrophobic Cluster Analysis (HCA), which has been useful for the prediction of orthologous proteins in different eukaryotic lineages [ 29, 30].
Although protocols for sequencing cpDNA based on consensus primer walking of total genomic DNA exist [18], it is still essential to have protocols based on the more conventional approach of sequencing chloroplast DNA via highly purified cpDNA because it allows efficient detection of SNPs and insertion-deletion polymorphisms (Indels) within a plant population.
The SNPs selected for validation indicated that the approach of sequencing cDNA would be beneficial for generating new set of DNA markers in sorghum.
The combined approach of 3' sequencing and computational identification of alternative polyadenylation ensured that the final array represented all detectable 3' splice/polyadenylation variants identified by our methods.
Complete Genomics' approach of sequencing by unchained ligation uses anchor probes to find adaptor sites and detection probes consisting of nine bases (nonamers) and four dyes.
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