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Several Ang structures are available in the databases, including three belonging to mice (with accession numbers 2J4T, 2BWL and 2BWK) and many other belonging to synthetic versions of humans or resulting from different approaches of crystallization.
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To obtain X-ray-grade crystals we have used the recently proposed approach of crystallization by mutational surface entropy reduction, in which selected Lys → Ala, Glu → Ala, and/or combined point mutations are introduced into the target protein to reduce the cumulative conformational entropy of surface residues.
Approach of crystallization in space using counter diffusion method has been conducted in order to reduce buoyancy-driven convection and avoid sedimentation of crystals.
According to this model the separation factor approaches the ratio of the equilibrium constants at small rates of crystallization and approaches the ratio of the forward rate constants at large rates of crystallization.
The glass forming ability of the alloys has been evaluated on the basis of the avoidance of crystallization approach.
We discuss the conceptual problems, and review strengths and limitations of current approaches towards crystallization prediction, emphasizing the importance of comprehensive and valid sampling protocols.
Advances in simulation and control algorithms and process sensor technologies have enabled the development of systematic first-principles and direct design approaches for the batch control of crystallization processes.
A new approach is proposed for the study of crystallization processes under the hypothesis of isokinetic behavior.
An approach based on a 2-step nucleation mechanism of crystallization may be used to interpret the observations of polymer crystallization around and in the glass transition region.
Availability of structural data is the main limitation of this approach given that crystallization of large multisubunit protein complexes, especially full-size CRLs, can be notoriously difficult.
In conclusion, the in vitro in silico approach can be used for predictions of absorption effects of crystallization, but the model could benefit from further development work on the theoretical crystallization model and in vitro experimental design.
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CEO of Professional Science Editing for Scientists @ prosciediting.com