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Previous approaches for tagging neurons in vivo have been based mainly on imaging methods typically two photon microscopy for recognizing GFP expression or application of calcium dependent indicators, which can be expressed in specific cells types and allow the observation of activity in specified neuronal populations [12], [39].
To generate high producer cell clones, different approaches for tagging chromosomal loci were evaluated.
The present study focuses on the evaluation of different approaches for tagging and the evaluation of the flexibility of incoming cassettes concerning the predictability of expression upon targeting.
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The efficient intracellular expression of these functional molecules opens up the possibility of developing an alternative approach for tagging intracellular targets with fluorescent proteins for a range of molecular cell biology imaging studies.
Phenix et al. used an analogue of [F]FDG in an enzymatic approach for tagging acid β-glucocerebrosidase (GCase), a recombinant enzyme formulated in Cerezyme which is used to treat Gaucher disease [ 42].
In contrast to previously developed tools, which are typically restricted to identifying genes mentions in the literature, the paper by C.-H. Wei et al. entitled "GNormPlus: An Integrative Approach for Tagging Genes, Gene Families, and Protein Domains" implemented an open source system that is able to detect genes, gene families, and protein domains.
This approach is similar to the one used for tagging the Sentiment 140 Tweet Corpus.
Thus, of all potential capture techniques, the purse seine (as used here) is likely the best approach for collecting, tagging, and biopsying fish for selectivity experiments.
Healthcare professionals, in collaboration with laypeople, should prepare and share bookmark lists of useful web resources concerning health issues or adopt a social bookmarking approach for discovering, tagging, sharing, rating and recommending relevant web resources, using vocabulary and terminology that can be understood by all.
Herein, we consider a mono-static approach for tag localization.
Based on FCA, [26] presented a novel approach for tag recommendation based on users' interest lattice matching (UILM).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com