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Therefore, alternative approaches for sequence comparisons are preferable to further characterize these genes.
Also, for a very large superfamily (>20 members), the BRS-PHI-BLAST will be a good option to choose, however the choice of any of the discussed approaches for sequence search becomes superfamily specific.
In order to get the accurate information about the CNVs and excluded false positives, clone-ordered-based approaches for sequence assembly and further annotation of repeats are needed in further study.
The MEA framework has been shown to be very effective in constructing accurate alignment of multiple biological sequences, making it one of the most popular approaches for sequence alignment.
Thus, our results suggest that, at present, clone-ordered-based approaches for sequence assembly appear to be a more effective resolution for identifying the true locations, organization and complexity of SDs.
The amount of sequence produced has also provided the computational challenge of developing accurate and reliable software approaches for sequence alignment that can complete withina useful time frame using affordable computer hardware resources.
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To this end, common approaches for sequencing ribosomal peptides using tandem mass spectrometry are not applicable to NRPs.
Two approaches for sequencing were compared.
Traditional approaches for sequencing them have required grams of tissue to extract and enrich sufficient quantities of 'pure' mtDNA (e.g. [ 19]).
Nearly all traditional (Sanger) [ 1- 3, 8, 11- 15] and still some NGS [ 16] approaches for sequencing mitogenomes rely on long range PCR amplification.
The recent development of high-throughput approaches for sequencing and annotating mitochondrial genomes [ 22] holds great promise for increasing the representation of nematode mtDNA genomes for analysis, and has already yielded several new strongylid sequences analyzed herein.
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